Evaluation of the antimicrobial effectiveness of 222 nm UV-C light as a treatment strategy for the preservation of biological specimens in liquid from the “José Ricardo Cure Hakim” Collection at Universidad Militar Nueva Granada
Abstract
Biological collections are records of biodiversity. However, biodeterioration threatens their conservation, making evaluating treatments that mitigate the associated damage crucial. This article assessed the antimicrobial effectiveness of 222nm UV light against microorganisms on the skin of vertebrate specimens preserved in liquid from the "Jose Ricardo Cure Hakim" collection at Universidad Militar Nueva Granada, Cajicá, Colombia. Microorganisms were obtained by swabbing, isolated, and identified molecularly and morphologically. In vitro, antimicrobial activity was evaluated with microorganisms on glass beads at four time points. After irradiation, percentage inhibition was calculated. The effect on specimens was analyzed by swabbing. Bacillus and Paenibacillus genera were identified. 222nm UV light showed an inhibitory effect on bacterial growth in vitro and on specimens. The findings suggest new strategies to mitigate biodeterioration in liquid biological collections.
Downloads
References
ALHARBI, M., BAKITIAN, F., & ALENZI, A. (2021). “Evaluation of bactericidal effects of ultraviolet light C irradiation on cariogenic bacteria: An in vitro study”. BMC Oral Health, 21(1). https://doi.org/10.1186/s12903-021-01767-x.
ARBELÁEZ-CORTÉS, E., ACOSTA-GALVIS, A., DONASCIMIENTO, C., GONZÁLEZ ALVARADO, A., MEDINA, C. (2017). “Knowledge linked to museum specimen vouchers: measuring scientific production from a major biological collection in Colombia”. Scientometrics, 112(3): 1323–1341. https://doi.org/10.1007/s11192-017-2461-4.
ARENAS-CASTRO, H., MUÑOZ-GOMEZ, S. A., URIBE-ACOSTA, M., CASTANO-CASTANO, L., LIZARAZO-MEDINA, P. (2016). “Richness cellulolytic activity, and fungicide susceptibility of fungi from a bird biological collection”. Acta biológica colombiana. https://repositorio.unal.edu.co/handle/unal/61227.
BRANYSOVA, T., DEMNEROVA, K., DUROVIC, M., & STIBOROCA, H. (2022). “Microbial biodeterioration of cultural heritage and identification 2of the active agents over the last two decades”. In Journal of Cultural Heritage 55: 245–260). https://doi.org/10.1016/j.culher.2022.03.013.
BUONANNO, M., PONNAIYA, B., WELCH, D., STANISLAUSKAS, M., RANDERS-PEHRSON, G., SMINELOV, L., LOWY, F., OWENS, D., BRENNER, D. (2017). “Germicidal efficacy and mammalian skin safety of 222-nm UV light”. Radiation Research, 187(4): 483–491. https://doi.org/10.1667/RR0010CC.1.
CARRILLO CHAVEZ, L. M., ARIAS BERMÚDEZ, N., NIETO VANEGAS, M. J., PINTO SANCHEZ, N. R., & PATIÑO, M. C. (2023). “Study of biodeterioration and evaluation of proteolytic activity caused by microorganisms in specimens of a zoological collection in Colombia”. Conservar Patrimonio. https://doi.org/10.14568/cp24895.
CERSOY, S., HERBIN, M. (2022). “Wet collections: preserving their values for the future! Heritage for the Future, Science for Heritage, A European Adventure for Research and Innovation”. HAL open science. 157–169. https://hal.science/hal-03889249v1.
CUTLER, T., ZIMMERMAN, J. (2011). “Ultraviolet irradiation and the mechanisms underlying its inactivation of infectious agents”. In Animal health research reviews .12(1): 15–23. https://doi.org/10.1017/S1466252311000016.
DOMÁNSKI, J., JANCZURA, A., WANAT, M., WIGLUSZ, K., GRAJZER, M., SIMMONS, J.E., DOMAGALA, Z., & SZEPIETOWSKI, J. C. (2023). “Preservation fluids of heritage anatomical specimens —a challenge for modern science. Studies of the origin, composition and microbiological contamination of old museum collections”. Journal of Anatomy. https://doi.org/10.1111/joa.13854.
FAHEEM, F. (2019). “Toxic effect of myristica fragrans essential oil against the museum pest Anthrenus verbasci (coleoptera: dermestidae) to control biodeterioration of animal collections”. Journal of Bio Innovation, 8(5): 554–571. https://doi.org/10.1038/NPROT.2008.92.
FUKUDA, S., NISHIKAWA, J., KOBAYASHI, Y., OKABE, Y., FUJII, T., KUBOTA, N., OTSUYAMA, K-I., TSUNEOKA, H., HASHIMOTO, S., SUEHIRO. Y., YAMASAKI, T., &
TAKAMI, T. (2022). “The bactericidal effect of far-UVC on ESBL-producing Escherichia coli”. American Journal of Infection Control, 50(11): 1268–1270. https://doi.org/10.1016/j.ajic.2022.04.012
GÓRNY, R. L., GOLOFIT-SZYMCZAK, M., PALAK, A., LAWNICKEK-WALCZYK, A., CYPROWSKI, M., STOBNICKA, A., PLOCINSKA, M., & KOWALSKA, J. (2024). “Effectiveness of UV-C radiation in inactivation of microorganisms on materials with different surface structures”. Annals of Agricultural and Environmental Medicine, 31(2): 287–293. https://doi.org/10.26444/aaem/189695
HILTON, E., WATKINS-COLWELL, G., HUBER, S. (2021). “The Expanding Role of Natural History Collections”. Ichthyology and Herpetology, 109(2): 379–391. https://doi.org/10.1643/t2020018.
IENES-LIMA, J., PRICHULA, J., ABADIE, M. (2023). “First Report of Culturable Skin Bacteria in Melanophryniscus admirabilis (Admirable Redbelly Toad)”. Microb Ecol 86: 756–761. https://doi.org/10.1007/s00248-022-02069-7.
KAKAKHEL, M.A., WU, F., GU, J. D., FENG, H., SHAH, K., & WANG, W. (2019). “Controlling biodeterioration of cultural heritage objects with biocides: A review.” In International Biodeterioration and Biodegradation,143. Elsevier Ltd. https://doi.org/10.1016/j.ibiod.2019.104721.
KAMPF, G. (2018). “Ethanol. In Antiseptic Stewardship”. Springer International Publishing. MDPI. 9-35. https://doi.org/10.3390/books978-3-7258-2915-6.
KATARA, G., HEMVANI, N., CHITNIS, S., CHITNIS, V., & CHITNIS, D. S. (2008). Surface disinfection by exposure to germicidal UV light. Indian Journal of Medical Microbiology, 26(3): 241–242. https://doi.org/10.4103/0255-0857.42034.
KONRAT, K., SCHWEBKE, I., LAUE, M., DITTMANN, C., LEVIN, K., ET AL. (2016). “The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants”. PLOS ONE 11(6): e0157663. https://doi.org/10.1371/journal.pone.0157663
LATORRE, J.D, HERNANDEZ-VELASCO, X., WOLFENDEN, R. E., VICENTE, J.L., WOLFENDEN, A. D., MENCONI, A., BIELKE, L., HARGIS, B.M., & TLLEZ, G. (2016). “Evaluation and selection of Bacillus species based on enzyme production, antimicrobial activity, and biofilm synthesis as direct-fed microbial candidates for poultry”. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2016.00095
LINNIE, M, J. (2000). “Prevention of biodeterioration in natural history collections: Potential trends and future developments”. In Museum Management and Curatorship. Taylor & Francis. 18(3): 295–300). https://doi.org/10.1080/09647770000501803
MEINEKE, E., DAVIES, T., DARU, B., DABIS, C. (2019). “Biological collections for understanding biodiversity in the Anthropocene”. In Philosophical Transactions of the Royal Society B: Biological Sciences. https://doi.org/10.1098/rstb.2017.0386
MOHAPATRA, S., YUTAO, L., GOH, S. G., NG, C., LUHUA, Y., TRAN, N. H., & GIN, K. Y. H. (2023). Quaternary ammonium compounds of emerging concern: Classification, occurrence, fate, toxicity and antimicrobial resistance”. Journal of Hazardous Materials, 3-10. https://doi.org/10.1016/j.jhazmat.2022.130393.
MUÑOZ, MORENO, G., &-VERRI. (2011). “Estandarización de un protocolo de caracterización molecular para la identificación de especie de cepas terrestres de bacterias del género Brucella”. Revista Sapuvet de Salud Pública. https://revistas.lasalle.edu.co/index.php/us/article/view/5086.
NARITA, K., ASANO, K., NAITO, K., OHASHI, H., SASAKI, M., MORIMOTO, Y., IGIRAHI, T., & NAKANE, A. (2020). “Ultraviolet C light with wavelength of 222nm inactivates a wide spectrum of microbial pathogens”. Journal of Hospital Infection. https://doi.org/10.1016/j.jhin.2020.03.030.
NEISSKENWIRTH, F., & TROXLER, M. (2021). Biocides and other harmful substances on dry and fluid preserved specimens, 63-72. https://doi.org/10.11588/arthistoricum.1179.c16172
NEGI, A., & SARETHY, I. P. (2019). “Microbial Biodeterioration of Cultural Heritage: Events, Colonization, and Analyses”. Microbial Ecology, 78(4): 1014–1029. https://doi.org/10.1007/s00248-019-01366-y
NGUYET, A., FURUTANI, N., ANDER, R., IZAWA, S. (2022). “Acquired resistance to severe ethanol stress-induced inhibition of proteasomal proteolysis in Saccharomyces cerevisiae”. Biochimica et Biophysica Acta. https://doi.org/10.1016/j.bbagen.2022.130241
NING, P., HAN, Y., LIU, Y., LIU, S., SUN, Z., WANG, X., WANG, B., GAO, F., WANG, Y., WANG, Y., GAO, X., CHEN, G., & LI, X. (2023). “Study on disinfection effect of a 222nm UVC excimer lamp on object surface”. AMB Express. https://doi.org/10.1186/s13568-023-01611-1
NISHIGORI, C., YAMANO, N., KUNISADA, M., NISHIAKI-SAWADA, A., OHASHI, H., & IGARASHI, T. (2023). “Biological Impact of Shorter Wavelength Ultraviolet Radiation-C”. In Photochemistry and Photobiology 99(2): 335–343. https://doi.org/10.1111/php.13742
PIOUS, T. (2011). “Long-Term Survival of Bacillus Spores in Alcohol and Identification of 90% Ethanol as Relatively More Spori/Bactericidal”. Current Microbiology. https://doi.org/10.1007/s00284-011-0040-0
PROENÇA, D. N., FASOLA, E., LOPES, I., MORAIS, P. V. (2021). “Characterization of the Skin Cultivable Microbiota Composition of the Frog Pelophylax perezi Inhabiting Different Environments”. Int J Environ Res Public Health. https://doi.org/10.3390/ijerph18052585
SABLIER, M., DAVRON, C., & HERBIN, M. (2020). “Characterization of the JAR Sealants in the Fluid Collections of the Muséum National D’histoire Naturelle”. Collection Forum, 34(1): 87–100. https://doi.org/10.14351/0831-4985-34.1.87
SAWYER, S. F. (2009). “Analysis of Variance: The Fundamental Concepts”. Journal of Manual & Manipulative Therapy, 17(2): 27E-38E. https://doi.org/10.1179/jmt.2009.17.2.27E.
SIMMONS, J. E., & MUÑOZ-SABA, YANETH. (2005). “Cuidado, manejo y conservación de las colecciones biológicas”. Universidad Nacional de Colombia: Conservación Internacional. https://www.researchgate.net/publication/266249611_Cuidado_Manejo_y_Conservacion_de_las_Colecciones_Biologicas
SIMMONS, J.E. (2014). “Fluid Preservation: A Comprehensive Reference”. Enviromental Science. https://doi.org/10.5771/9781442229662
TAVARES, R. S. N. ADAMOSKI, D., GIRASOLE, A., LIMA, E. N., DA SILVA JUSTO-JUNIOR, A., DOMINGUES, R., SILVEIRA, A.C.C., MARQUES, R. E., DE CARVALHO, M.,
AMROSIO, A. L. B., LEME, A. F. P., & DIAS, S.M.G. (2023). “Different biological effects of exposure to far-UVC (222nm) and near-UVC (254nm) irradiation”. Journal of Photochemistry and Photobiology. https://doi.org/10.1016/j.jphotobiol.2023.112713.
TRUJILLO, E., TRIVIÑO, P., SALAZAR, L. (2014). “Clasificación, manejo y conservación de colecciones biológicas: una mirada a su importancia para la biodiversidad”. Momentos de Ciencia, 11(2) ,97–106.https://www.researchgate.net/publication/305682393_Clasificacion_manejo_y_conservacion_de_colecciones_biologicas_una_mirada_a_su_importancia_para_la_biodiversidad
WEN, J., ICKERT-BOND, S. M., APPELHANS, M. S., DORR, L. J., & FUNK, V. A. (2015). “Collections-based systematics: Opportunities and outlook for 2050”. Journal of Systematics and Evolution, 53(6): 477–488. https://doi.org/10.1111/jse.12181
WEIHS, P., SCHMALWIESER, A. W., & SCHAUBERGER, G. (2012). “UV Effects on Living Organisms”. Environmental Toxicology, 609–688. https://doi.org/10.1007/978-1-4614-5764-0_22
XIE, R., TSE, G. Y. K., MAN, L. C. L., CHEUNG, A. L. H., WONG, D. T. C., LAM, E. H. M., HUANG, H., & LEUNG, D. Y. C. (2022). “Systematical Investigations on Disinfection Effectiveness of Far-UVC (222 nm) irradiation: From Laboratory Study to Field Tests”. Journal of Applied & Environmental Microbiology, 10(1): 17–34. https://pubs.sciepub.com/jaem/10/1/3
YAMANO, N., KUNISADA, M., KAIDZU, S., SUGIHARA, K., NISHIAKI-SAWADA, A., OHASHI, H., YOSHIOKA, A., IGARASHI, T., OHIRA, A., TANITO, M., & NISHIGORI, C. (2020). “Long-term Effects of 222nm ultraviolet radiation C Sterilizing Lamps on Mice Susceptible to Ultraviolet Radiation”. Photochemistry and Photobiology, 96(4): 853–862. https://doi.org/10.1111/php.13269
YEATES, D., ZWICK, A., MIKHEYEV, A. (2016). “Museums are biobanks: unlocking the genetic potential of the three billion specimens in the world’s biological collections”. Insect Science, 18: 83–88. https://doi.org/10.1016/j.cois.2016.09.009
- Copyright and intellectual property belongs to author. Author guarantees editing and publishing rights to Ge-Conservación Journal, under a Creative Commons Attribution License. This license allows others to share the work with authorship and the original source of publication acknowledgement.
- Articles can be used for scientific and educational purposes but never for commercial use, being sanctioned by law.
- The whole content of the article is author’s responsibility.
- Ge-Conservación Journal and authors may establish additional agreements for non-exclusive distribution of the work version published at the Journal (for example, on institutional repositories or on a book) with acknowledgment of the original publication on this Journal.
- Author is allowed and encouraged to disseminate his works electronically (for example, on institutional repositories or on its own website) after being published on Ge-Conservación Journal. This will contribute for fruitful interchanges as also for wider and earlier citations of the author’s works.
- Author’s personal data will only be used for the Journal purposes and will not be given to others.
