Avaliação da eficácia antimicrobiana da luz UV-C de 222 nm como estratégia de tratamento para a preservação de espécimes biológicos em líquido da Coleção “José Ricardo Cure Hakim” da Universidade Militar Nueva Granada

Palavras-chave: coleções biológicas, biodeterioro, microrganismos, Bacillus, Paenibacillus

Resumo

As coleções biológicas constituem registos de biodiversidade. Contudo, a biodeterioração representa uma ameaça à sua conservação, sendo, por isso, essencial avaliar tratamentos que mitiguem os danos associados. Este artigo avaliou a eficácia antimicrobiana da luz UV de 222 nm face a microrganismos presentes na pele de espécimes de vertebrados preservados em líquido da coleção “José Ricardo Cure Hakim”, da Universidade Militar Nueva Granada, em Cajicá, Colômbia. Os microrganismos foram obtidos por zaragatoa, isolados e identificados molecular e morfologicamente. A atividade antimicrobiana in vitro foi avaliada com microrganismos sobre esferas de vidro em quatro intervalos de tempo. Após a irradiação, calcularam-se os percentuais de inibição. O efeito sobre os exemplares foi analisado através de zaragatoas. Identificaram-se géneros de Bacillus e Paenibacillus. A luz UV de 222 nm demonstrou um efeito inibitório sobre o crescimento bacteriano in vitro e nos exemplares. Os resultados sugerem novas estratégias para mitigar a biodeterioração em coleções biológicas conservadas em líquido.

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Biografias Autor

Maria Alejandra Gonzalez Torres, Estudiante de Facultad de Ciencias Básicas y Aplicadas Universidad Militar Nueva Granada Cajicá, Colombia

Bióloga de la Universidad Militar Nueva Granada, con experiencia en microbiología, conservación de colecciones científicas y análisis estadístico de datos biológicos. Ha participado en investigaciones sobre el uso de luz ultravioleta para la desinfección de especímenes biológicos, así como en el estudio de pigmentos bacterianos con propiedades antimicrobianas. Interesada en la aplicación de herramientas microbiológicas para la conservación del patrimonio biológico. Se distingue por su responsabilidad, pensamiento crítico y capacidad para trabajar en equipo.

Nelsy Rocio Pinto Sanchez, Facultad de Ciencias Básicas y Aplicadas Universidad Militar Nueva Granada Cajicá, Colombia

Bióloga, docente con especial interés en procesos de diversificación en la región Neotropical. Curadora de la colección zoológica “José Ricardo Cure Hakim” de la Universidad Militar Nueva Granada, Cajicá, Colombia. Interesada en el mantenimiento de las colecciones zoológicas.

Referências

ALHARBI, M., BAKITIAN, F., & ALENZI, A. (2021). “Evaluation of bactericidal effects of ultraviolet light C irradiation on cariogenic bacteria: An in vitro study”. BMC Oral Health, 21(1). https://doi.org/10.1186/s12903-021-01767-x.

ARBELÁEZ-CORTÉS, E., ACOSTA-GALVIS, A., DONASCIMIENTO, C., GONZÁLEZ ALVARADO, A., MEDINA, C. (2017). “Knowledge linked to museum specimen vouchers: measuring scientific production from a major biological collection in Colombia”. Scientometrics, 112(3): 1323–1341. https://doi.org/10.1007/s11192-017-2461-4.

ARENAS-CASTRO, H., MUÑOZ-GOMEZ, S. A., URIBE-ACOSTA, M., CASTANO-CASTANO, L., LIZARAZO-MEDINA, P. (2016). “Richness cellulolytic activity, and fungicide susceptibility of fungi from a bird biological collection”. Acta biológica colombiana. https://repositorio.unal.edu.co/handle/unal/61227.

BRANYSOVA, T., DEMNEROVA, K., DUROVIC, M., & STIBOROCA, H. (2022). “Microbial biodeterioration of cultural heritage and identification 2of the active agents over the last two decades”. In Journal of Cultural Heritage 55: 245–260). https://doi.org/10.1016/j.culher.2022.03.013.

BUONANNO, M., PONNAIYA, B., WELCH, D., STANISLAUSKAS, M., RANDERS-PEHRSON, G., SMINELOV, L., LOWY, F., OWENS, D., BRENNER, D. (2017). “Germicidal efficacy and mammalian skin safety of 222-nm UV light”. Radiation Research, 187(4): 483–491. https://doi.org/10.1667/RR0010CC.1.

CARRILLO CHAVEZ, L. M., ARIAS BERMÚDEZ, N., NIETO VANEGAS, M. J., PINTO SANCHEZ, N. R., & PATIÑO, M. C. (2023). “Study of biodeterioration and evaluation of proteolytic activity caused by microorganisms in specimens of a zoological collection in Colombia”. Conservar Patrimonio. https://doi.org/10.14568/cp24895.

CERSOY, S., HERBIN, M. (2022). “Wet collections: preserving their values for the future! Heritage for the Future, Science for Heritage, A European Adventure for Research and Innovation”. HAL open science. 157–169. https://hal.science/hal-03889249v1.

CUTLER, T., ZIMMERMAN, J. (2011). “Ultraviolet irradiation and the mechanisms underlying its inactivation of infectious agents”. In Animal health research reviews .12(1): 15–23. https://doi.org/10.1017/S1466252311000016.

DOMÁNSKI, J., JANCZURA, A., WANAT, M., WIGLUSZ, K., GRAJZER, M., SIMMONS, J.E., DOMAGALA, Z., & SZEPIETOWSKI, J. C. (2023). “Preservation fluids of heritage anatomical specimens —a challenge for modern science. Studies of the origin, composition and microbiological contamination of old museum collections”. Journal of Anatomy. https://doi.org/10.1111/joa.13854.

FAHEEM, F. (2019). “Toxic effect of myristica fragrans essential oil against the museum pest Anthrenus verbasci (coleoptera: dermestidae) to control biodeterioration of animal collections”. Journal of Bio Innovation, 8(5): 554–571. https://doi.org/10.1038/NPROT.2008.92.

FUKUDA, S., NISHIKAWA, J., KOBAYASHI, Y., OKABE, Y., FUJII, T., KUBOTA, N., OTSUYAMA, K-I., TSUNEOKA, H., HASHIMOTO, S., SUEHIRO. Y., YAMASAKI, T., &

TAKAMI, T. (2022). “The bactericidal effect of far-UVC on ESBL-producing Escherichia coli”. American Journal of Infection Control, 50(11): 1268–1270. https://doi.org/10.1016/j.ajic.2022.04.012

GÓRNY, R. L., GOLOFIT-SZYMCZAK, M., PALAK, A., LAWNICKEK-WALCZYK, A., CYPROWSKI, M., STOBNICKA, A., PLOCINSKA, M., & KOWALSKA, J. (2024). “Effectiveness of UV-C radiation in inactivation of microorganisms on materials with different surface structures”. Annals of Agricultural and Environmental Medicine, 31(2): 287–293. https://doi.org/10.26444/aaem/189695

HILTON, E., WATKINS-COLWELL, G., HUBER, S. (2021). “The Expanding Role of Natural History Collections”. Ichthyology and Herpetology, 109(2): 379–391. https://doi.org/10.1643/t2020018.

IENES-LIMA, J., PRICHULA, J., ABADIE, M. (2023). “First Report of Culturable Skin Bacteria in Melanophryniscus admirabilis (Admirable Redbelly Toad)”. Microb Ecol 86: 756–761. https://doi.org/10.1007/s00248-022-02069-7.

KAKAKHEL, M.A., WU, F., GU, J. D., FENG, H., SHAH, K., & WANG, W. (2019). “Controlling biodeterioration of cultural heritage objects with biocides: A review.” In International Biodeterioration and Biodegradation,143. Elsevier Ltd. https://doi.org/10.1016/j.ibiod.2019.104721.

KAMPF, G. (2018). “Ethanol. In Antiseptic Stewardship”. Springer International Publishing. MDPI. 9-35. https://doi.org/10.3390/books978-3-7258-2915-6.

KATARA, G., HEMVANI, N., CHITNIS, S., CHITNIS, V., & CHITNIS, D. S. (2008). Surface disinfection by exposure to germicidal UV light. Indian Journal of Medical Microbiology, 26(3): 241–242. https://doi.org/10.4103/0255-0857.42034.

KONRAT, K., SCHWEBKE, I., LAUE, M., DITTMANN, C., LEVIN, K., ET AL. (2016). “The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants”. PLOS ONE 11(6): e0157663. https://doi.org/10.1371/journal.pone.0157663

LATORRE, J.D, HERNANDEZ-VELASCO, X., WOLFENDEN, R. E., VICENTE, J.L., WOLFENDEN, A. D., MENCONI, A., BIELKE, L., HARGIS, B.M., & TLLEZ, G. (2016). “Evaluation and selection of Bacillus species based on enzyme production, antimicrobial activity, and biofilm synthesis as direct-fed microbial candidates for poultry”. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2016.00095

LINNIE, M, J. (2000). “Prevention of biodeterioration in natural history collections: Potential trends and future developments”. In Museum Management and Curatorship. Taylor & Francis. 18(3): 295–300). https://doi.org/10.1080/09647770000501803

MEINEKE, E., DAVIES, T., DARU, B., DABIS, C. (2019). “Biological collections for understanding biodiversity in the Anthropocene”. In Philosophical Transactions of the Royal Society B: Biological Sciences. https://doi.org/10.1098/rstb.2017.0386

MOHAPATRA, S., YUTAO, L., GOH, S. G., NG, C., LUHUA, Y., TRAN, N. H., & GIN, K. Y. H. (2023). Quaternary ammonium compounds of emerging concern: Classification, occurrence, fate, toxicity and antimicrobial resistance”. Journal of Hazardous Materials, 3-10. https://doi.org/10.1016/j.jhazmat.2022.130393.

MUÑOZ, MORENO, G., &-VERRI. (2011). “Estandarización de un protocolo de caracterización molecular para la identificación de especie de cepas terrestres de bacterias del género Brucella”. Revista Sapuvet de Salud Pública. https://revistas.lasalle.edu.co/index.php/us/article/view/5086.

NARITA, K., ASANO, K., NAITO, K., OHASHI, H., SASAKI, M., MORIMOTO, Y., IGIRAHI, T., & NAKANE, A. (2020). “Ultraviolet C light with wavelength of 222nm inactivates a wide spectrum of microbial pathogens”. Journal of Hospital Infection. https://doi.org/10.1016/j.jhin.2020.03.030.

NEISSKENWIRTH, F., & TROXLER, M. (2021). Biocides and other harmful substances on dry and fluid preserved specimens, 63-72. https://doi.org/10.11588/arthistoricum.1179.c16172

NEGI, A., & SARETHY, I. P. (2019). “Microbial Biodeterioration of Cultural Heritage: Events, Colonization, and Analyses”. Microbial Ecology, 78(4): 1014–1029. https://doi.org/10.1007/s00248-019-01366-y

NGUYET, A., FURUTANI, N., ANDER, R., IZAWA, S. (2022). “Acquired resistance to severe ethanol stress-induced inhibition of proteasomal proteolysis in Saccharomyces cerevisiae”. Biochimica et Biophysica Acta. https://doi.org/10.1016/j.bbagen.2022.130241

NING, P., HAN, Y., LIU, Y., LIU, S., SUN, Z., WANG, X., WANG, B., GAO, F., WANG, Y., WANG, Y., GAO, X., CHEN, G., & LI, X. (2023). “Study on disinfection effect of a 222nm UVC excimer lamp on object surface”. AMB Express. https://doi.org/10.1186/s13568-023-01611-1

NISHIGORI, C., YAMANO, N., KUNISADA, M., NISHIAKI-SAWADA, A., OHASHI, H., & IGARASHI, T. (2023). “Biological Impact of Shorter Wavelength Ultraviolet Radiation-C”. In Photochemistry and Photobiology 99(2): 335–343. https://doi.org/10.1111/php.13742

PIOUS, T. (2011). “Long-Term Survival of Bacillus Spores in Alcohol and Identification of 90% Ethanol as Relatively More Spori/Bactericidal”. Current Microbiology. https://doi.org/10.1007/s00284-011-0040-0

PROENÇA, D. N., FASOLA, E., LOPES, I., MORAIS, P. V. (2021). “Characterization of the Skin Cultivable Microbiota Composition of the Frog Pelophylax perezi Inhabiting Different Environments”. Int J Environ Res Public Health. https://doi.org/10.3390/ijerph18052585

SABLIER, M., DAVRON, C., & HERBIN, M. (2020). “Characterization of the JAR Sealants in the Fluid Collections of the Muséum National D’histoire Naturelle”. Collection Forum, 34(1): 87–100. https://doi.org/10.14351/0831-4985-34.1.87

SAWYER, S. F. (2009). “Analysis of Variance: The Fundamental Concepts”. Journal of Manual & Manipulative Therapy, 17(2): 27E-38E. https://doi.org/10.1179/jmt.2009.17.2.27E.

SIMMONS, J. E., & MUÑOZ-SABA, YANETH. (2005). “Cuidado, manejo y conservación de las colecciones biológicas”. Universidad Nacional de Colombia: Conservación Internacional. https://www.researchgate.net/publication/266249611_Cuidado_Manejo_y_Conservacion_de_las_Colecciones_Biologicas

SIMMONS, J.E. (2014). “Fluid Preservation: A Comprehensive Reference”. Enviromental Science. https://doi.org/10.5771/9781442229662

TAVARES, R. S. N. ADAMOSKI, D., GIRASOLE, A., LIMA, E. N., DA SILVA JUSTO-JUNIOR, A., DOMINGUES, R., SILVEIRA, A.C.C., MARQUES, R. E., DE CARVALHO, M.,

AMROSIO, A. L. B., LEME, A. F. P., & DIAS, S.M.G. (2023). “Different biological effects of exposure to far-UVC (222nm) and near-UVC (254nm) irradiation”. Journal of Photochemistry and Photobiology. https://doi.org/10.1016/j.jphotobiol.2023.112713.

TRUJILLO, E., TRIVIÑO, P., SALAZAR, L. (2014). “Clasificación, manejo y conservación de colecciones biológicas: una mirada a su importancia para la biodiversidad”. Momentos de Ciencia, 11(2) ,97–106.https://www.researchgate.net/publication/305682393_Clasificacion_manejo_y_conservacion_de_colecciones_biologicas_una_mirada_a_su_importancia_para_la_biodiversidad

WEN, J., ICKERT-BOND, S. M., APPELHANS, M. S., DORR, L. J., & FUNK, V. A. (2015). “Collections-based systematics: Opportunities and outlook for 2050”. Journal of Systematics and Evolution, 53(6): 477–488. https://doi.org/10.1111/jse.12181

WEIHS, P., SCHMALWIESER, A. W., & SCHAUBERGER, G. (2012). “UV Effects on Living Organisms”. Environmental Toxicology, 609–688. https://doi.org/10.1007/978-1-4614-5764-0_22

XIE, R., TSE, G. Y. K., MAN, L. C. L., CHEUNG, A. L. H., WONG, D. T. C., LAM, E. H. M., HUANG, H., & LEUNG, D. Y. C. (2022). “Systematical Investigations on Disinfection Effectiveness of Far-UVC (222 nm) irradiation: From Laboratory Study to Field Tests”. Journal of Applied & Environmental Microbiology, 10(1): 17–34. https://pubs.sciepub.com/jaem/10/1/3

YAMANO, N., KUNISADA, M., KAIDZU, S., SUGIHARA, K., NISHIAKI-SAWADA, A., OHASHI, H., YOSHIOKA, A., IGARASHI, T., OHIRA, A., TANITO, M., & NISHIGORI, C. (2020). “Long-term Effects of 222nm ultraviolet radiation C Sterilizing Lamps on Mice Susceptible to Ultraviolet Radiation”. Photochemistry and Photobiology, 96(4): 853–862. https://doi.org/10.1111/php.13269

YEATES, D., ZWICK, A., MIKHEYEV, A. (2016). “Museums are biobanks: unlocking the genetic potential of the three billion specimens in the world’s biological collections”. Insect Science, 18: 83–88. https://doi.org/10.1016/j.cois.2016.09.009

Publicado
2025-10-27
Como Citar
Gonzalez Torres, M. A., & Pinto Sanchez, N. R. (2025). Avaliação da eficácia antimicrobiana da luz UV-C de 222 nm como estratégia de tratamento para a preservação de espécimes biológicos em líquido da Coleção “José Ricardo Cure Hakim” da Universidade Militar Nueva Granada. Ge-Conservacion, 28(1), 121-131. https://doi.org/10.37558/gec.v28i1.1404